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s200 gel filtration column  (Bio-Rad)


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    Bio-Rad s200 gel filtration column
    S200 Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3948 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s200 gel filtration column/product/Bio-Rad
    Average 96 stars, based on 3948 article reviews
    s200 gel filtration column - by Bioz Stars, 2026-05
    96/100 stars

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    (A) SDS-PAGE of POPs samples from affinity chromatography experiments: 1= Protein MW ladder (kDa), 2= Trypanosoma cruzi POP ( Tc POP), 3= Trypanosoma brucei POP ( Tb POP), 4= Leishmania major POP ( Li POP) and Homo sapiens POP ( Hu POP). SEC-MALS experiments, using a <t>S200</t> 10/300 confirmed the theoretical molecular mass values for each POP (B-E) of ∼ 78 kDa, corresponding to ∼ 13 mL elution volume.
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    (A) SDS-PAGE of POPs samples from affinity chromatography experiments: 1= Protein MW ladder (kDa), 2= Trypanosoma cruzi POP ( Tc POP), 3= Trypanosoma brucei POP ( Tb POP), 4= Leishmania major POP ( Li POP) and Homo sapiens POP ( Hu POP). SEC-MALS experiments, using a S200 10/300 confirmed the theoretical molecular mass values for each POP (B-E) of ∼ 78 kDa, corresponding to ∼ 13 mL elution volume.

    Journal: bioRxiv

    Article Title: Open and closed conformations of a sub-80 kDa Chagas vaccine candidate defined by a cryo-EM led integrative approach

    doi: 10.1101/2024.03.26.586384

    Figure Lengend Snippet: (A) SDS-PAGE of POPs samples from affinity chromatography experiments: 1= Protein MW ladder (kDa), 2= Trypanosoma cruzi POP ( Tc POP), 3= Trypanosoma brucei POP ( Tb POP), 4= Leishmania major POP ( Li POP) and Homo sapiens POP ( Hu POP). SEC-MALS experiments, using a S200 10/300 confirmed the theoretical molecular mass values for each POP (B-E) of ∼ 78 kDa, corresponding to ∼ 13 mL elution volume.

    Article Snippet: Finally, the proteins were concentrated using 30kDa MWCO Amicon (Millipore) ultra-centrifugal filters to 10 mg/mL and immediately injected in gel filtration column S200 10/300 (Cytiva) equilibrated with 20mM Hepes and 150mM NaCl at pH 7.4.

    Techniques: SDS Page, Affinity Chromatography